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Plants were grown in A. thaliana gene silencing suppressor (tobacco rattle virus; TRV) virus-induced gene-silencing (VIGS) leaves in four different treatments: P. syringae without MAMP (control); elf18 or flg22 without TRV; elf18 or flg22 with TRV and without MAMP; and elf18 or flg22 with TRV and with MAMP. In a second experiment, plants were grown in the same conditions, except that MAMP treatment was replaced by elf18 or flg22 expression using dexamethasone (DEX) with three different concentrations ( 2.5mM, 5mM and 10mM). The two MAMP treatments were paired with each of the three concentrations of DEX, creating four additional treatments. In both experiments, plants were grown and the severity of SGI was estimated as described in Vetter et al. [ 3 ]. MAMP-treated and non-treated plants were grown and compared to estimate the effect of MAMP on SGI. All plants were grown in 16 dishes; each dish contained two plants of the same genotype. In total, there were ~400 plants in each experiment.
By constructing a dozen of different sequence variants with a predetermined motif, we were able to reduce the possibility of spurious mapping in that region. The reduced sequence space allowed us to confidently map >3.5 million SNPs to the MAMP receptor locus. SNPs detected in the MAMP receptor locus for elf18 and flg22 treatments at 8-16 hpi were consistently present at a statistically significant level in the mid-parent line over all SGI loci. This suggests that these sequences might provide a different genetic background leading to differences in MAMP response. We also noted the existence of non-synonymous SNPs for elf18 and flg22 treatment within the receptor locus that might alter function.
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MAMP PRO is a local, commercial, of server environment local Classic: MAMP. Designed for web developers and professional programmers, MAMP PRO allows easy installation and environmental management of their development. MAMP PRO can publish the server your WordPress. Just enter the login information server directly into your remote tab, and select the database to move along with the root directory. Your project can go into operation with just a click of the mouse.
MAMP installs a local server environment in a matter of seconds on your macOS or Windows computer. MAMP comes free of charge, and is easily installed. MAMP will not compromise any existing Apache installation already running on your system.
MAMP is a command line tool and it’s very easy to setup and use. All you have to do is just type the mamp command in the terminal. Have a look at the mamp command usage and how to install MAMP on Windows or Mac. The main features of mamp are:
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PHP 5+ running on Windows or Mac.
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FastCGI/mod_fcgid running on Windows or Mac.
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MySQL 5+ on Windows or Mac.
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OpenSSL client certificate support on Windows or Mac.
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ModSecurity installed and configured by default.
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What’s new in MAMP PRO 5.0.5.3998?
MAC Address MAMP PRO now automatically detects the correct configuration for your MAC address. In MAMP PRO 5.0.5.3998, the MAC address database was improved. MAMP PRO is also more responsive to changes in the system. For example, if you change your local network, MAMP PRO will also take this into account.
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Using MAMP PRO, you can install WordPress and use the WordPress CLI to create a project and add pages. It is the only MAMP PRO cloud solution that allows you to create your own hosting while working on the cloud. You can also publish your page to any live server with the click of a button. This is a powerful feature and can be used for example, for submitting pages to crowdfunding sites.
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What’s new in MAMP PRO 5.0.5.3998
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